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Enteric Fermentation Base (Fermentation Base for Campylobacter)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Enteric Fermentation Base

(Fermentation Base for Campylobacter)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptic digest of animal tissue ...........................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

Carbohydrate solution................................. 100.0mL

Andrade’s indicator....................................... 10.0mL

pH 7.2 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 100.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Glucose, lactose, mannitol, sucrose, adonitol, arabi

nose, cellobiose, dulcitol, glycerol, inositol, salicin,

xylose, or other carbohydrates may be used. For the

preparation of expensive carbohydrate solutions

(adonitol, arabinose, cellobiose, dulcitol, glycerol,

inositol, salicin, or xylose), 5.0g of carbohydrate per

100.0mL of distilled/deionized water may be used.

Andrade’s Indicator:

Composition per 100.0mL:

NaOH (1N solution)...................................... 16.0mL

Acid Fuchsin.......................................................0.1g

Caution: Acid Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Preparation of Andrade’s Indicator: Add

components to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes

that contain an inverted Durham tube in 9.0mL vol

umes. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C. Aseptically add 1.0mL of ster

ile carbohydrate solution per tube. Mix thoroughly.

Use: For the cultivation and differentiation of a vari

ety of bacteria based on their ability to ferment differ

ent carbohydrates. Bacteria that produce acid from

carbohydrate fermentation turn the medium dark

pink to red. Bacteria that produce gas have a bubble

trapped in the Durham tube.

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Enteric Fermentation Base (Fermentation Base for Campylobacter)