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Entamoeba Medium (Endamoeba Medium)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Entamoeba Medium

(Endamoeba Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Liver infusion..................................................272.0g

Rice powder ......................................................14.2g

Agar ..................................................................11.0g

Proteose peptone.................................................5.5g

Sodium glycerophosphate...................................3.0g

NaCl....................................................................2.7g

Horse serum .................................................. 50.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systemss.

Rice Powder:

Composition per 15.0g:

Rice powder ......................................................15.0g

Preparation of Rice Powder: Sterilize rice pow

der at 160°C for 60 min. Do not overheat or rice pow

der will scorch.

Preparation of Medium: Add components, ex

cept horse serum and rice powder, to distilled/deion

ized water and bring volume to 994.0mL. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes in 7.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C. Allow tubes to cool in

a slanted position. Aseptically add enough sterile

horse serum to each tube to cover about half the slant.

Aseptically add 0.1g of sterile rice powder to each

tube.

Use: For the cultivation of Entamoeba histolytica.