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Xylophilus Medium

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

Xylophilus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

CaCO3 ......................................................................................... 20.0g

Agar ............................................................................................ 15.0g

D-Galactose ................................................................................. 10.0g

Yeast extract................................................................................ 10.0g

Ferric ammonium citrate solution............................................10.0mL

Ferric Ammonium Citrate Solution:

Composition per 10.0mL:

Ferric ammonium citrate............................................................. 0.25g

Preparation of Ferric Ammonium Citrate Solution: Add fer

ric ammonium citrate to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Preparation of Medium: Add components, except ferric ammoni

um citrate solution, to distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically

add 10.0mL of sterile ferric ammonium citrate solution. Mix thorough

ly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Xylophilus ampelina.