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Xylose Lactose Tergitol™ 4 (XLT-4)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

Xylose Lactose Tergitol™ 4

(XLT-4)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004.6mL:

Agar ............................................................................................ 18.0g

Lactose.......................................................................................... 7.5g

Sucrose.......................................................................................... 7.5g

Na2S2O3 ........................................................................................ 6.8g

Lysine............................................................................................ 5.0g

NaCl.............................................................................................. 5.0g

Xylose......................................................................................... 3.75g

Yeast extract.................................................................................. 3.0g

Proteose peptone........................................................................... 1.6g

Ferric ammonium citrate............................................................... 0.8g

Phenol Red.................................................................................. 0.08g

Selective supplement solution ...................................................4.6mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Selective Supplement Solution:

Composition per 100.0mL:

Tergitol™ 4........................................................................ Proprietary

Preparation of Selective Supplement Solution: Available as a

premixed solution.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Add 4.6mL of selective supplement solution.

Mix thoroughly. Gently heat while stirring and bring to boiling. Do not

autoclave. Cool to 50°C. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the isolation and identification of salmonellae from clinical,

environmental, and food samples. The presence of the selective agent,

Tergitol™ 4, in this medium inhibits many organisms that can be prob

lematic on other plating media. In addition, biochemical and pH

changes within the medium allow Salmonella spp. (black colonies) to

be differentiated from organisms such as E. coli (yellow colonies) and

Shigella spp. (red colonies). The enhanced selectivity of XLT-4 Agar

reduces the need for further identification procedures, saving time and

money, and results in fewer false presumptive positive colonies when

compared to other Salmonella plating media.