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Xylella fastidiosa Medium (LMG 115)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

Xylella fastidiosa Medium

(LMG 115)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 17.0g

Yeast extract................................................................................ 10.0g

ACES buffer................................................................................ 10.0g

Activated charcoal ........................................................................ 2.0g

L-Cysteine-iron solution...........................................................20.0mL

pH 6.9 ± 0.2 at 25°C

L-Cysteine-Iron Solution:

Composition per 20.0mL:

L-Cysteine·HCl.............................................................................. 0.4g

Fe4(P2O7)3 ................................................................................... 0.25g

Preparation of L-Cysteine-Iron Solution: Add components to

distilled/deionized water and bring volume to 20.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add ACES to 500.0mL of distilled/de

ionized water at 50°C. Add a solution containing 40.0mL of 1N KOH

in 440.0mL of distilled water. Mix thoroughly. Add the remaining

components, except L-cysteine-iron solution. Mix thoroughly. Gently

heat and bring to boiling. Adjust pH to 6.9 with KOH. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add

20.0mL of sterile L-cysteine-iron solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Xylella fastidiosa.