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Xylella Agar (LMG Medium 115)

[所属分类:培养基配方] [发布时间:2021-10-15] [发布人:网站管理员2] [阅读次数:] [返回]

Xylella Agar

(LMG Medium 115)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 17.0g

Yeast extract................................................................................ 10.0g

ACES .......................................................................................... 10.0g

Activated charcoal ........................................................................ 2.0g

α-ketoglutarate.............................................................................. 1.0g

KOH, 1N.....................................................................................40mL

L-Cysteine-iron solution ..........................................................20.0mL

pH 6.9 ± 0.2 at 25°C

L-Cysteine-Iron Solution:

Composition per 20.0mL:

L-Cysteine·HCl.............................................................................. 0.4g

Fe4(P2O7)3 ................................................................................... 0.25g

Preparation of L-Cysteine-Iron Solution: Add components to

distilled/deionized water and bring volume to 20.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add ACES to 500.0mL of distilled water

at 50°C. Combine with a solution containing 40.0mL of 1N KOH in

440.0mL of distilled water. Add the other components except cysteine

iron solution. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically

add 20.0mL sterile cysteine iron solution. Pour into sterile Petri dishes

or distribute into sterile tubes.

Use: For the cultivation of Xylella spp.