培养基配方

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Wilson and Blair’s Medium

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 165.0mL:

Nutrient agar solution ............................................................100.0mL

Solution B ................................................................................45.0mL

Solution A................................................................................20.0mL

pH 6.8 ± 0.2 at 25°C

Nutrient Agar Solution:

Composition per 100.0mL:

Agar ............................................................................................ 1.95g

Pancreatic digest of gelatin......................................................... 0.65g

Beef extract................................................................................. 0.39g

Preparation of Nutrient Agar Solution: Aseptically add compo

nents to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Solution A:

Composition per liter:

Na2HPO4................................................................................... 100.0g

Na2SO3...................................................................................... 100.0g

Glucose ....................................................................................... 50.0g

Bismuth ammonium citrate......................................................... 30.0g

Preparation of Solution A: Aseptically add 30.0g of bismuth am

monium citrate to 250.0mL of boiling distilled/deionized water. Asep

tically add Na2SO3 to 500.0mL of boiling distilled/deionized water.

Mix the two solutions. Add the Na2HPO4 to the boiling mixture. Cool

to 45°C. In a separate flask, aseptically add glucose to 250.0mL of ster

ile distilled/deionized water. Mix thoroughly. Aseptically add the glu

cose solution to the other cooled solution.

Solution B:

Composition per 225.0mL:

Ferric citrate.................................................................................. 2.0g

Brilliant Green ............................................................................ 0.55g

Preparation of Solution B: Aseptically add 2.0g of ferric citrate to

200.0mL of sterile distilled/deionized water. Mix thoroughly. Asepti

cally add Brilliant Green to 25.0mL of sterile distilled/deionized water.

Mix thoroughly. Aseptically combine the two solutions.

Preparation of Medium: Aseptically combine 100.0mL of nutrient

agar solution, 20.0mL of solution A, and 45.0mL of solution B. Mix

thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Clostridium welchii.