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Wilson Blair Base

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wilson Blair Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 30.0g

Proteose peptone No. 3............................................................... 10.0g

Glucose ....................................................................................... 10.0g

Beef extract................................................................................... 5.0g

NaCl.............................................................................................. 5.0g

Selective reagent ......................................................................70.0mL

Brilliant Green (1% solution) ....................................................4.0mL

pH 7.3 ± 0.2 at 25°C

Selective Reagent:

Composition per 320.2mL:

Solution 1...............................................................................100.0mL

Solution 2...............................................................................100.0mL

Solution 3...............................................................................100.0mL

Solution 4.................................................................................20.2mL

Preparation of Selective Reagent: Combine 100.0mL of solution

1, 100.0mL of solution 2, 100.0mL of solution 3, and 20.2mL of solu

tion 4. Mix thoroughly. Gently heat to boiling until a slate-grey color

develops. Cool to 50°C.

Solution 1:

Composition per 100.0mL:

NaHSO3 ...................................................................................... 40.0g

Preparation of Solution 1: Add NaHSO3 to 100.0mL of distilled/

deionized water. Mix thoroughly.

Solution 2:

Composition per 100.0mL:

NaH2PO4 ..................................................................................... 21.0g

Preparation of Solution 2: Add NaH2PO4 to 100.0mL of distilled/

deionized water. Mix thoroughly.

Solution 3:

Composition per 100.0mL:

Bismuth ammonium citrate......................................................... 12.5g

Preparation of Solution 3: Add bismuth ammonium citrate to

100.0mL of distilled/deionized water. Mix thoroughly.

Solution 4:

Composition per 20.2mL:

FeSO4 .......................................................................................... 0.96g

Preparation of Solution 4: Add FeSO4 to 20.0mL of distilled/de

ionized water. Add 0.2mL of concentrated HCl. Mix thoroughly.

Preparation of Medium: Add components, except selective re

agent and Brilliant Green solution, to distilled/deionized water and

bring volume to 976.0mL. Mix thoroughly. Gently heat and bring to

boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add selective reagent and

Brilliant Green solution. Mix thoroughly. Pour into sterile Petri dishes

or leave in tubes.

Use: For the isolation and cultivation of Salmonella, especially Salmo

nella typhi.

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Wilson Blair Base