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Wilkins-Chalgren Anaerobic HiVeg Broth Base with Blood

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wilkins-Chalgren Anaerobic HiVeg Broth Base

with Blood

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate......................................................................... 10.0g

Plant peptone............................................................................... 10.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 5.0g

Sodium pyruvate ........................................................................... 1.0g

Glucose ......................................................................................... 1.0g

L-Arginine ..................................................................................... 1.0g

Fe4(P2O7)3·H2O..........................................................................5.0mg

Menadione .................................................................................0.5mg

Defibrinated blood ...................................................................50.0mL

pH 7.1 ± 0.2 at 25°C

Source: This medium, without blood, is available as a premixed pow

der from HiMedia.

Preparation of Medium: Add components, except defibrinated

blood, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C. Aseptically add 50.0mL of defibrinated blood. Mix thoroughly.

Aseptically distribute into tubes or leave in flasks.

Use: For the cultivation of nonsporulating anaerobes. For the cultivation

and maintenance of anaerobic bacteria. For standardized antimicrobic

susceptibility testing to determine the minimum inhibitory concentra

tions of antimicrobics for anaerobic bacteria.