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Wilkins-Chalgren Anaerobic HiVeg Agar Base with Blood and Nonspore Anaerobic Supplement

[所属分类:培养基配方] [发布时间:2021-10-13] [发布人:网站管理员2] [阅读次数:] [返回]

Wilkins-Chalgren Anaerobic HiVeg Agar Base

with Blood and Nonspore Anaerobic Supplement

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

Plant hydrolysate......................................................................... 10.0g

Plant peptone............................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Yeast extract.................................................................................. 5.0g

L-Arginine ..................................................................................... 1.0g

Glucose ......................................................................................... 1.0g

Sodium pyruvate ........................................................................... 1.0g

Fe4(P2O7)3·H2O..........................................................................5.0mg

Menadione .................................................................................0.5mg

Defibrinated blood ...................................................................50.0mL

Nonspore anaerobic supplement..........................................................

pH 7.1 ± 0.2 at 25°C

Source: This medium, without blood or nonspore anaerobic supple

ment, is available as a premixed powder from HiMedia.

Nonspore Anaerobic Supplement:

Composition per 10.0mL:

Sodium pyruvate ........................................................................... 1.0g

Nalidixic acid...........................................................................10.0mg

Ferric pyrophosphate, soluble....................................................5.0mg

Menadione .................................................................................0.5mg

Preparation of Nonspore Anaerobic Supplement: Add com

ponents to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, except defibrinated

blood and nonspore anaerobic supplement, to distilled/deionized water

and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of de

fibrinated blood and 10.0mL nonspore anaerobic supplement. Mix

thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of nonsporulating anaerobes. For the cultivation

and maintenance of anaerobic bacteria. For standardized antimicrobic

susceptibility testing to determine the minimum inhibitory concentra

tions of antimicrobics for anaerobic bacteria.