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W Medium

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

W Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur .......................................................................................... 10.0g

KH2PO4......................................................................................... 3.0g

MgSO4·7H2O ................................................................................ 0.5g

CaCl2·2H2O................................................................................. 0.25g

(NH4)2SO4..................................................................................... 0.2g

FeSO4·7H2O.............................................................................10.0mg

pH 3.0 ± 0.2 at 25°C

Preparation of Sulfur: Sterilize by steaming at 100°C for 60 min

on 3 consecutive days.

Preparation of Medium: Add components, except sulfur, to dis

tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0g

of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of Thiobacillus thiooxidans.