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Wagatsuma Agar

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

Wagatsuma Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

NaCl ............................................................................................ 70.0g

Agar ............................................................................................ 15.0g

Mannitol...................................................................................... 10.0g

Peptone........................................................................................ 10.0g

K2HPO4 ........................................................................................ 5.0g

Yeast extract.................................................................................. 3.0g

Crystal Violet .............................................................................1.0mg

Red blood cells.........................................................................50.0mL

pH 8.0 ± 0.2 at 25°C

Red Blood Cells:

Composition per 100.0mL:

Blood, human or rabbit ..........................................................100.0mL

Preparation of Red Blood Cells: Mix freshly drawn human or

rabbit blood with anticoagulant and an equal volume of sterile 0.85%

saline solution. Centrifuge cells at 4000 × g at 4°C for 15 min. Pour off

saline and wash two more times with sterile saline. After last wash,

pour off saline and resuspend cells to their original volume.

Preparation of Medium: Add components, except blood, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 8.0. Place in a steam bath for 30 min. Do not autoclave. Cool

to 45°–50°C. Add 50.0mL of washed red blood cells. Mix thoroughly.

Pour into sterile Petri dishes. Dry plates before using.

Use: For the cultivation and detection of thermostable hemolysin of

Vibrio parahaemolyticus by the Kanagawa reaction.