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VY/4-SWS Agar (DSMZ Medium 958)

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VY/4-SWS Agar

(DSMZ Medium 958)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

NaCl............................................................................................ 20.0g

Agar ............................................................................................ 15.0g

Yeast cell paste (baker’s yeast, washed in

deionized water) wet weight .................................................. 2.5g

Seawater salts solution..................................................................1.0L

Vitamin B12 solution ..................................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Seawater Salts Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 8.0g

CaCl2·2H2O .................................................................................. 1.0g

KCl................................................................................................ 0.5g

NaHCO3...................................................................................... 0.16g

KBr ............................................................................................. 0.08g

SrCl2·6H2O ................................................................................. 0.03g

H3BO3 ......................................................................................... 0.02g

Ferric citrate................................................................................ 0.01g

di-Na-ß-glycerophosphate .......................................................... 0.01g

Trace elements solution SL-4 ....................................................1.0mL

Trace Elements Solution SL-4:

Composition per liter:

EDTA............................................................................................ 0.5g

FeSO4·7H2O.................................................................................. 0.2g

Trace elements solution SL-6 ................................................100.0mL

Preparation of Trace Elements Solution SL-4: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Elements Solution SL-6:

Composition per liter:

H3BO3 ........................................................................................... 0.3g

CoCl2·6H2O .................................................................................. 0.2g

ZnSO4·7H2O ................................................................................. 0.1g

MnCl2·4H2O ............................................................................... 0.03g

Na2MoO4·H2O ............................................................................ 0.03g

NiCl2·6H2O................................................................................. 0.02g

CuCl2··2H2O................................................................................ 0.01g

Preparation of Trace Elements Solution SL-6: Add components

to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Adjust pH to 3.4.

Preparation of Seawater Salts Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Vitamin B12 Solution:

Composition per 10.0mL:

Cyanocobalamin ........................................................................ 5.0mg

Preparation of Vitamin B12 Solution: Add cyanocobalamine to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Sparge with 100% N2. Filter sterilize.

Preparation of Medium: Add NaCl, agar, and yeast cell paste to

1.0L seawater salts solution. Mix thoroughly. Adjust pH to 7.5 with 1M

NaOH. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi

pressure–121°C. Cool to 50°C. Aseptically add 1.0mL sterile vitamin

B12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in

tubes.

Use: For the cultivation and maintenance of Haliangium ochraceum,

Haliangium tepidum, Plesiocystis pacifica, and Enhygromyxa salina

(Thaxtera salina).

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VY/4-SWS Agar (DSMZ Medium 958)