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VRE Agar

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VRE Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Tryptone...................................................................................... 20.0g

Agar ............................................................................................ 10.0g

Yeast extract.................................................................................. 5.0g

NaCl.............................................................................................. 5.0g

Sodium citrate............................................................................... 1.0g

Esculin .......................................................................................... 1.0g

Ferric ammonium citrate............................................................... 0.5g

NaN3 ........................................................................................... 0.15g

Selective supplement solution ...................................................4.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Selective Supplement Solution:

Composition per 4.0mL:

Meropenum................................................................................ 1.0mg

Vancomycin ............................................................................... 6.0mg

Preparation of Selective Supplement Solution: Add components

to distilled/deionized water and bring volume to 4.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asep

tially add 4.0mL selective supplement solution. Mix thoroughly . Pour

into sterile Petri dishes.

Use: For the isolation of vancomycin resistant enterococci (VRE)

from clinical samples. Nonresistant enterococci containing the Van C

genes will not grow on this medium.The selective supplement sup

presses growth of Gram-negative bacteria and E. gallinarum. The

medium contains an indicator system to detect the growth of esculin

hydrolyzing organisms. Enterococci produce black zones around the

colonies from the formation of black iron phenolic compounds derived

from esculin-hydrolyis products and ferrous iron.