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VRB MUG Agar (Violet Red Bile Lactose MUG Agar)

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VRB MUG Agar

(Violet Red Bile Lactose MUG Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.0g

Lactose........................................................................................ 10.0g

Meat peptone ................................................................................ 7.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Bile salts mixture .......................................................................... 1.5g

4-Methylumbelliferyl-β-D-glucuronide ........................................ 0.1g

Neutral Red................................................................................. 0.03g

Crystal Violet............................................................................ 0.002g

pH 7.4 ± 0.2 at 37°C

Source: This medium is available from Fluka, Sigma-Aldrich.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Pour into sterile Petri dishes.

Use: For the detection and enumeration of coliform bacteria, in partic

ular E. coli. Gram-positive accompanying flora are extensively inhib

ited by Crystal Violet and bile salts. A color change to red indicates lac

tose-positive colonies, within which E. coli can be demonstrated by

fluorescence in the UV.