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VRE Agar

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VRE Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Tryptone...................................................................................... 20.0g

Agar ............................................................................................ 10.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 5.0g

Sodium citrate ............................................................................... 1.0g

Esculin .......................................................................................... 1.0g

Ferric ammonium citrate............................................................... 0.5g

NaN3 ........................................................................................... 0.15g

Selective supplement solution ...................................................4.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

Selective Supplement Solution:

Composition per 4.0mL:

Gentamicin.............................................................................512.0mg

Preparation of Selective Supplement Solution: Add gentamicin

to distilled/deionized water and bring volume to 4.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except selective sup

plement solution, to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asep

tially add 4.0mL selective supplement solution. Mix thoroughly . Pour

into sterile Petri dishes.

Use: For the isolation of high-level aminoglycoside-resistant entero

cocci (HLARE) from clinical samples. Nonresistant enterococci con

taining the Van C genes will not grow on this medium.The selective

supplement suppresses growth of Gram-negative bacteria and E. galli

narum. The medium contains an indicator system to detect the growth

of esculin-hydrolyzing organisms. Enterococci produce black zones

around the colonies from the formation of black iron phenolic com

pounds derived from esculin hydrolyis products and ferrous iron.