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VRB Agar, Fluorocult (Fluorocult VRB Agar)

[所属分类:培养基配方] [发布时间:2021-10-12] [发布人:网站管理员2] [阅读次数:] [返回]

VRB Agar, Fluorocult

(Fluorocult VRB Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.0g

Lactose ........................................................................................ 10.0g

Peptone from meat ........................................................................ 7.0g

NaCl .............................................................................................. 5.0g

Yeast extract.................................................................................. 3.0g

Bile salts mixture .......................................................................... 1.5g

4-Methylumbelliferyl-β-D-glucuronide ........................................ 0.1g

Neutral Red ................................................................................. 0.03g

Crystal Violet ............................................................................ 0.002g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available from Merck.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Heat in a boiling wa

ter bath or in free flowing steam with frequent stirring until completely

dissolved. Do not boil for more than 2 min. Do not autoclave. Do not

overheat. Pour into sterile Petri dishes. The plates are clear and dark

red.

Use: For the detection and enumeration of coliform bacteria, in partic

ular E. coli. Crystal Violet and bile salts largely inhibit the growth of

Gram-positive accompanying bacterial flora. Lactose-positive colo

nies show a color change to red of the pH indicator. E. coli colonies

show a fluorescence under UV light. Lactose-negative Enterobacteri

aceae are colorless. Lactose-positive colonies are red and often sur

rounded by a turbid zone due to the precipitation of bile acids. Light

blue fluorescing colonies denote E. coli.