Anaerobic Trypticase ™ Soy Agar with Calf Blood (ATCC Medium 1664)

[所属分类:培养基配方] [发布时间:2019-10-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic Trypticase ™ Soy Agar with Calf Blood

(ATCC Medium 1664)
Composition per liter:
Pancreatic digest of casein..........................................................15.0g
Agar............................................................................................15.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Calf blood, defibrinated.........................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except calf blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Prepare medium anaerobically with 80% N 2 + 10% CO 2 + 10% H 2 .
Gently heat while stirring and bring to boiling for 1 min. Autoclave for
15 min at 15 psi pressure–121°C. Do not overheat. Cool to 45°–50°C.
Aseptically add 100.0mL sterile, defibrinated calf blood. Pour into
sterile Petri dishes.
Use: For the isolation and cultivation of fastidious as well as nonfas-
tidious microorganisms. For the differentiation of Haemophilus spe-
cies.