Anaerobic Thioglycollate Medium Base with Serum

[所属分类:培养基配方] [发布时间:2019-10-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic Thioglycollate Medium Base with Serum
Composition per liter:
Casein enzymic hydrolysate.......................................................17.0g
Meat extract..................................................................................7.5g
D -Glucose......................................................................................6.0g
Liver hydrolysate..........................................................................3.0g
Papaic digest of soybean meal......................................................3.0g
NaCl..............................................................................................2.5g
Agar..............................................................................................0.7g
Sodium thioglycollate...................................................................0.5g
L -Cysteine...................................................................................0.25g
Na 2 SO 3 ..........................................................................................0.1g
Serum, sterile.........................................................................100.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except serum, to distilled/
deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust
pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–
55°C. Aseptically add 100.0mL of sterile serum. Mix thoroughly. Asepti-
cally distribute into sterile tubes.
Use: For the selective isolation of anaerobic bacteria.