Anaerobic CNA Agar Base with Blood

[所属分类:培养基配方] [发布时间:2019-10-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Composition per liter:
Agar............................................................................................13.5g
Casein enzymic hydrolysate.......................................................12.0g
Peptic digest of animal tissue .......................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................3.0g
Corn starch....................................................................................1.0g
Glucose.........................................................................................1.0g
L -Cystine hydrochloride ...............................................................0.5g
Dithiothreitol (DTE).....................................................................0.1g
Vitamin K 1 ..................................................................................0.01g
Hemin .........................................................................................0.01g
Colistin........................................................................................0.01g
Nalidixic acid..............................................................................0.01g
Sheep blood, sterile defibrinated.............................................50.0mL
pH 7.0 ± 0.2 at 25°C

Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 50.0mL of sterile sheep blood. Mix thor-
oughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation of anaerobic streptococci.