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Anaerobic CNA Agar (Anaerobic Colistin Nalidixic Acid Agar)

[所属分类:培养基配方] [发布时间:2019-10-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic CNA Agar
(Anaerobic Colistin Nalidixic Acid Agar)
Composition per liter:
Agar............................................................................................13.0g
Pancreatic digest of casein..........................................................12.0g
Peptic digest of animal tissue .......................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Beef extract...................................................................................3.0g
Cornstarch.....................................................................................1.0g
Glucose.........................................................................................1.0g
L- Cysteine·HCl·H 2 O .....................................................................0.5g
Vitamin K 1 ...............................................................................10.0mg
Hemin ......................................................................................10.0mg
Colistin.....................................................................................10.0mg
Nalidixic acid...........................................................................10.0mg
Sheep blood, defibrinated........................................................50.0mL
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL of sterile,
defibrinated sheep blood. Mix thoroughly. Pour into sterile Petri dish-
es.
Use: For the selective isolation of anaerobic streptococci.

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