AN1 Medium

[所属分类:培养基配方] [发布时间:2019-10-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AN1 Medium
Composition per liter:
Pancreatic digest of casein..........................................................10.0g
Sulfur, powdered...........................................................................8.0g
NaCl..............................................................................................2.5g
K 2 HPO 4 .........................................................................................1.5g
Disodium thioglycolate solution...................................................1.0g
Resazurin ...................................................................................1.0mg
pH 7.3 ± 0.2 at 25°C
Disodium Thioglycolate Solution:
Composition per 10.0mL:
Disodium thioglycolate.................................................................1.0g
Preparation of Disodium Thioglycolate Solution: Add disodi-
um thioglycolate to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except powdered sulfur
and disodium thioglycolate solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Al-
low to cool under 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C. Powdered sulfur is sterilized separately by steaming for 3 hr on 3
consecutive days. Aseptically and anaerobically combine the basal solu-
tion, sterile powdered sulfur, and sterile disodium thioglycolate solution
under 100% N 2 .
Use:  For the cultivation of Thermococcus species.