Amygdalin Medium

[所属分类:培养基配方] [发布时间:2019-10-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Amygdalin Medium
Composition per liter:
Peptone .......................................................................................10.0g
Beef extract...................................................................................5.0g
NaCl..............................................................................................5.0g
Agar..............................................................................................3.0g
Amygdalin solution ...............................................................200.0mL
Bromthymol Blue (0.05% solution) ..........................................5.0mL
pH 7.0 ± 0.2 at 25°C
Amygdalin Solution:
Composition per 200.0mL:
Amygdalin..................................................................................10.0g
Preparation of Amygdalin Solution: Add amygdalin to distilled/
deionized water and bring volume to 200.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except amygdalin so-
lution, to distilled/deionized water and bring volume to 800.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0. Auto-
clave for 20 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile amygdalin solution. Mix thoroughly. Aseptically
distribute into sterile tubes with cotton plugs. Allow tubes to cool in a
slanted position, forming a short slant.
Use: For the cultivation and differentiation of Serratia species based
on their ability to produce acid and HCN from amygdalin.