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Streptosel™ Agar

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

Streptosel™ Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 15.0g

Agar ............................................................................................ 12.0g

Glucose ......................................................................................... 5.0g

Papaic digest of soybean meal ...................................................... 5.0g

NaCl .............................................................................................. 4.0g

Sodium citrate ............................................................................... 1.0g

L-Cystine ....................................................................................... 0.2g

NaN3.............................................................................................. 0.2g

Na2SO3 .......................................................................................... 0.2g

Crystal Violet .............................................................................0.2mg

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Caution: Sodium azide is toxic. Azides also react with metals and

disposal must be highly diluted.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. If medium is used the same

day, do not autoclave. Pour into sterile Petri dishes or leave in tubes. If

medium is to be stored, autoclave for 15 min at 13 psi pressure–118°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For the selective isolation, cultivation, and enumeration of strep

tococci from specimens containing a mixed flora.