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Streptomycin Terramycin® Malt Extract Agar

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Malt extract................................................................................. 30.0g

Agar ............................................................................................ 15.0g

Peptone ......................................................................................... 5.0g

Streptomycin solution............................................................100.0mL

Terramycin solution ...............................................................100.0mL

pH 5.4 ± 0.2 at 25°C

Streptomycin Solution:

Composition per 100.0mL:

Streptomycin............................................................................... 0.07g

Preparation of Streptomycin Solution: Add streptomycin to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize.

Terramycin Solution:

Composition per 100.0mL:

Terramycin .................................................................................. 0.07g

Preparation of Terramycin Solution: Add terramycin to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except streptomycin

solution and terramycin solution, to distilled/deionized water and bring

volume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add 100.0mL of sterile streptomycin solution and 100.0mL

of sterile terramycin solution. Mix thoroughly. Pour into sterile Petri

dishes in 20.0mL volumes.

Use: For the cultivation and enumeration of fungi isolated from sew

age and polluted waters.