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Streptococcus agalactiae Selective HiVeg Agar Base with Blood and Staphylococcus B toxin

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Streptococcus agalactiae Selective HiVeg Agar Base

with Blood and Staphylococcus B toxin

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 13.0g

Plant peptone .............................................................................. 10.0g

NaCl.............................................................................................. 5.0g

Plant extract No. 1 ........................................................................ 5.0g

Esculin .......................................................................................... 1.0g

Thallous sulfate......................................................................... 0.333g

Crystal Violet................................................................................ 1.3g

Sheep blood, defibrinated ........................................................50.0mL

Staphylococcus B toxin............................................................25.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium, without blood or toxin, is available as a pre

mixed powder from HiMedia.

Preparation of Medium: Add components, except sheep blood and

staphylococcal toxin, to distilled/deionized water and bring volume to

925.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL of sterile sheep blood and 25.0mL of staphylococcal toxin.

Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.

Use: For the selective cultivation of Streptococcus agalactiae.