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Streptococcal Growth Medium

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................ 500.0g

Tryptose ...................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Glucose ......................................................................................... 1.0g

Bromcresol Purple solution .......................................................1.0mL

pH 7.4 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ...................................................................... 0.16g

Ethanol (95% solution)............................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol

Purple to 10.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

in 5.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Streptococcus species and other Gram-pos

itive cocci. Growth in this medium turns the indicator yellow and the

solution turbid.