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STAA Agar Base

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

STAA Agar Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................ 20.0g

Agar ............................................................................................ 13.0g

Glycerol ........................................................................................ 7.5g

Yeast extract.................................................................................. 2.0g

K2HPO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 1.0g

STAA selective supplement.....................................................10.0mL

pH 7.0 ± 0.1 at 25°C

Source: This medium is available as a premixed powder from Oxoid

Unipath.

STAA Selective Supplement:

Composition per 10.0mL:

Streptomycin sulfate ..................................................................... 0.5g

Cycloheximide ............................................................................ 0.05g

Thallous acetate .......................................................................... 0.05g

Preparation of STAA Selective Supplement: Add components

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except glycerol and

STAA selective supplement, to distilled/deionized water and bring vol

ume to 985.0mL. Mix thoroughly. Gently heat and bring to boiling.

Add glycerol. Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add sterile STAA selective

supplement. Mix thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation of Brochothrix thermosphacta from meat prod

ucts.