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StA (Schmitthenner's Agar)

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

StA

(Schmitthenner's Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 2.75g

NaNO3 ........................................................................................ 0.35g

KH2PO4....................................................................................... 0.15g

K2HPO4....................................................................................... 0.15g

MgSO4 .......................................................................................... 0.1g

Sitosterol.................................................................................... 0.01g

Agar ............................................................................................ 20.0g

Preparation of Medium: Add components, in the order listed, to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of Pythium aphaniderma

tum, Pythium graminicola, Pythium myriotylum, Pythiumsylvaticum,

and Pythium ultimum.