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ST Holding Medium (m-ST Holding Medium)

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

ST Holding Medium

(m-ST Holding Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4......................................................................................... 3.0g

Tris(hydroxymethyl)aminomethane buffer................................... 3.0g

Sulfanilamide................................................................................ 1.5g

NaH2PO4·H2O............................................................................... 0.1g

Ethanol (95% solution)............................................................10.0mL

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Dissolve the sulfanilamide in the ethanol.

Add all components to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Distribute in 1.8mL volumes to sterile Petri dishes with tight-fitting

lids and an absorbent filter.

Use: For the cultivation and enumeration of coliform bacteria by the

delayed-incubation total coliform procedure. For use as a holding or

transport medium to keep coliform bacteria viable between sampling

and laboratory culture.