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SSM Agar

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

SSM Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A ..............................................................................850.0mL

Solution B ................................................................................50.0mL

Solution C ..............................................................................100.0mL

Solution A:

Composition per 850.0mL:

Agar, noble.................................................................................. 15.0g

Na2HPO4 ....................................................................................... 6.0g

KH2PO4......................................................................................... 3.0g

MgSO4·7H2O ................................................................................ 0.2g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 850.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution B:

Composition per 50.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Solution B: Add glucose to distilled/deionized wa

ter and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°–55°C.

Solution C:

Composition per 100.0mL:

Casein hydrolysate...................................................................... 20.0g

Preparation of Solution C: Add casein hydrolysate to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

through Whatman #1 paper. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°–55°C.

Preparation of Medium: Aseptically combine 850.0mL of sterile

solution A, 50.0mL of sterile solution B, and 100.0mL of sterile solu

tion C. Mix thoroughly. Pour into sterile Petri dishes or aseptically dis

tribute into sterile tubes.

Use: For the cultivation and maintenance of a variety of bacteria.