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SPYE Medium (LMG Medium 262)

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

SPYE Medium

(LMG Medium 262)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sea salts....................................................................................... 30.0g

Agar No. 1................................................................................... 12.0g

Peptone.......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

Vitamin solution.........................................................................5.0mL

Glucose solution ........................................................................2.0mL

pH 7.3 ± 0.2 at 25°C

Vitamin Solution:

Composition per 10.0mL:

Riboflavin ..................................................................................2.0mg

Preparation of Vitamin Solution: Add riboflavin to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except vitamin solution

and glucose solution, to 993.0mL distilled/deionized water. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL of ster

ile vitamin solution and 2.0mL of sterile glucose solution. Mix thor

oughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Maricaulis spp.