培养基配方

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SPS HiVeg Agar, Modified

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant hydrolysate......................................................................... 15.0g

Yeast extract................................................................................ 10.0g

Na2SO3 ......................................................................................... 0.5g

Ferric citrate.................................................................................. 0.5g

Sulphadiazine.............................................................................. 0.12g

Na-thioglycollate .......................................................................... 0.1g

Sorbitan monooleate ................................................................... 0.05g

Polymyxin B sulfate.................................................................... 0.01g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 13 psi pressure–118°C. Pour into sterile Petri dishes or

leave in tubes.

Use: For the isolation and detection of Clostridium perfringens and

Clostridium botulinum in foods and other materials.