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Sporosarcina ureae Medium

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 30.0g

Glucose ......................................................................................... 4.0g

(NH4)2SO4..................................................................................... 4.0g

Malt extract................................................................................... 3.0g

Peptone ......................................................................................... 3.0g

Yeast extract.................................................................................. 2.0g

K2HPO4......................................................................................... 1.0g

MgSO4 .......................................................................................... 0.8g

CaCl2............................................................................................. 0.1g

MnSO4·H2O.................................................................................. 0.1g

CuSO4·5H2O............................................................................... 0.01g

ZnSO4 ......................................................................................... 0.01g

FeSO4·7H2O............................................................................... 1.0mg

Preparation of Medium: Add components to 1.0L of distilled/de

ionized water. Mix thoroughly. Gently heat and bring to boiling. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and induction of sporulation of Sporosarcina

ureae.