培养基配方

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Sporosarcina ureae Medium

[所属分类:培养基配方] [发布时间:2021-8-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

L-Asparagine·H2O or L-glutamine............................................... 30.0g

KCl................................................................................................ 3.4g

NaCl ............................................................................................ 2.92g

K2HPO4....................................................................................... 0.25g

(NH4)2SO4..................................................................................... 0.2g

MgSO4·7H2O .............................................................................. 0.05g

FeSO4·7H2O...............................................................................2.5mg

MnCl2·4H2O.............................................................................0.25mg

Biotin solution..........................................................................10.0mL

L-Cysteine solution ..................................................................10.0mL

(NH4)2SO4 solution..................................................................10.0mL

pH 8.7 ± 0.2 at 25°C

Biotin Solution:

Composition per 10.0mL:

D-Biotin ......................................................................................1.0mg

Preparation of Biotin Solution: Add biotin to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

L-Cysteine Solution:

Composition per 10.0mL:

L-Cysteine ................................................................................... 0.04g

Preparation of L-Cysteine Solution: Add L-cysteine to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

(NH4)2SO4 Solution:

Composition per 10.0mL:

(NH4)2SO4..................................................................................... 0.2g

Preparation of (NH4)2SO4 Solution: Add (NH4)2SO4 to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components—except biotin solu

tion, L-cysteine solution, and (NH4)2SO4 solution—to distilled/deion

ized water and bring volume to 970.0mL. Mix thoroughly. Adjust pH

to 8.7 with 1N NaOH. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add sterile biotin solution, L-cysteine

solution, and (NH4)2SO4 solution. Mix thoroughly. Aseptically distrib

ute into sterile tubes.

Use: For the cultivation of Sporosarcina ureae.