培养基配方

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Sporocytophaga Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaNO3........................................................................................... 2.0g

K2HPO4......................................................................................... 1.2g

MgSO4·7H2O ................................................................................ 1.0g

KCl................................................................................................ 0.5g

KH2PO4....................................................................................... 0.14g

Yeast extract................................................................................ 0.02g

FeSO4·7H2O...............................................................................6.0mg

Filter paper strips .................................................................... variable

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except filter paper

strips, to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min

at 15 psi pressure–121°C. Aseptically add a sterile filter paper strip to

each tube so that 1.0–2.0cm of the strip extends above the medium.

Use: For the cultivation and maintenance of Sporocytophaga myxo

coccoides.