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Sporobacter Medium (DSMZ Medium 711)

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Sporobacter Medium

(DSMZ Medium 711)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NH4Cl ........................................................................................... 1.0g

NaCl.............................................................................................. 0.6g

Na-acetate·3H2O........................................................................... 0.5g

Cysteine-HCl·H2O ........................................................................ 0.5g

K2HPO4......................................................................................... 0.3g

KH2PO4......................................................................................... 0.3g

Yeast extract.................................................................................. 0.2g

MgCl2·6H2O ................................................................................. 0.2g

CaCl2·2H2O .................................................................................. 0.1g

KCl............................................................................................... 0.1g

Resazurin ................................................................................... 0.5mg

NaHCO3 solution.....................................................................40.0mL

Trimethoxycinnamate solution ................................................10.0mL

Na2S·9H2O solution.................................................................10.0mL

Trace elements solution SL-10 ..................................................1.5mL

pH 7.1 ± 0.2 at 25°C

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O................................................................................... 1.5g

CoCl2·6H2O ........................................................................... 190.0mg

MnCl2·4H2O .......................................................................... 100.0mg

ZnCl2........................................................................................ 70.0mg

Na2MoO4·2H2O ....................................................................... 36.0mg

NiCl2·6H2O.............................................................................. 24.0mg

H3BO3 ........................................................................................ 6.0mg

CuCl2·2H2O ............................................................................... 2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly. Sparge with 80% N2 + 20% CO2. Autoclave for 15 min at 15

psi pressure–121°C.

Na2S·9H2O Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.3g

Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool

to room temperature.

NaHCO3 Solution:

Composition per 100.0mL:

NaHCO3...................................................................................... 10.0g

Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge

with 80% N2 + 20% CO2. Filter sterilize.

Trimethoxycinnamate Solution:

Composition per 10.0mL:

Trans-3,4,5-trimethoxycinnamate................................................. 1.2g

Preparation of Trimethoxycinnamate Solution: Add trans-

3,4,5-trimethoxycinnamate to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Neutralize with NaOH. Sparge with

100% N2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

room temperature.

Preparation of Medium: Prepare and dispense medium under 80%

N2 + 20% CO2 gas atmosphere. Add components, except NaHCO3 solu

tion, Na2S·9H2O solution, trimethoxycinnamate solution, and trace ele

ments solution SL-10, to distilled/deionized water and bring volume to

938.5mL. Mix thoroughly. Adjust pH to 7.1. Sparge with 80% N2 + 20%

CO2. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and an

aerobically add 40.0mL NaHCO3 solution, 10.0mL Na2S·9H2O solution,

10.0mL trimethoxycinnamate solution, and 1.5mL trace elements solution

SL-10. Mix thoroughly. Aseptically and anaerobically distribute into ster

ile tubes or bottles.

Use: For the cultivation of Sporobacter termitidis.

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Sporobacter Medium (DSMZ Medium 711)