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Spirochaeta halophila Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose salts solution ............................................................970.0mL

Yeast extract peptone solution .................................................30.0mL

Glucose Salts Solution:

Composition per liter:

NaCl ............................................................................................ 49.3g

MgSO4·7H2O .............................................................................. 49.2g

CaCl2·2H2O................................................................................... 5.9g

Glucose solution ....................................................................100.0mL

Sulfide solution ......................................................................100.0mL

Preparation of Glucose Salts Solution: Add components, except

glucose solution and sulfide solution, to distilled/deionized water and

bring volume to 800.0mL. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 25°C. Aseptically add sterile glucose so

lution and sulfide solution. Mix thoroughly.

Sulfide Solution:

Composition per 100.0mL:

Na2S·9H2O.................................................................................... 0.5g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Glucose Solution:

Composition per 100.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Yeast Extract Peptone Solution:

Composition per 30.0mL:

Yeast extract.................................................................................. 4.0g

Peptone.......................................................................................... 2.0g

Preparation of Yeast Extract Peptone Solution: Add compo

nents to distilled/deionized water and bring volume to 30.0mL. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to

25°C.

Preparation of Medium: Aseptically combine 30.0mL of yeast ex

tract peptone solution with 970.0mL of glucose salts solution. Mix

thoroughly. Aseptically distribute into sterile tubes or flasks.

Use: For the isolation and cultivation of Spirochaeta halophila.