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Spirochaeta aurantia Growth Medium

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract.................................................................................. 4.0g

Maltose.......................................................................................... 2.0g

Peptone.......................................................................................... 2.0g

Potassium phosphate

buffer (0.1M solution, pH 7.0).........................................100.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Filter sterilize potassium phosphate buf

fer. Add components, except potassium phosphate buffer, to distilled/

deionized water and bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile potassium phosphate

buffer. Mix thoroughly. Adjust pH to 7.2. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation of Spirochaeta aurantia.