培养基配方

您现在的位置：网站首页 >> 培养基配方

Spirochaeta aurantia Agar

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

Solution A.....................................................................................1.0L

Solution B ................................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Solution A:

Composition per liter:

Agar ............................................................................................ 10.0g

Pancreatic digest of casein............................................................ 5.0g

Glucose ......................................................................................... 2.0g

Yeast extract.................................................................................. 2.0g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5 with

KOH. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 50°–55°.

Solution B:

Composition per 200.0mL:

K2HPO4..................................................................................... 21.25g

KH2PO4..................................................................................... 10.62g

Preparation of Solution B: Add components to distilled/deion

ized water and bring volume to 200.0mL. Mix thoroughly. Adjust pH

to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–

55°C.

Preparation of Medium: Combine 1.0L of sterile solution A with

10.0mL of sterile solution B. Mix thoroughly. Aseptically pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Spirochaeta aurantia.