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Spirit Blue Agar

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

Spirit Blue Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Pancreatic digest of casein.......................................................... 10.0g

Yeast extract.................................................................................. 5.0g

Spirit Blue................................................................................... 0.15g

Lipoidal emulsion ....................................................................30.0mL

pH 6.8 ± 0.2 at 25°C

Lipoidal Emulsion:

Composition per 500.0mL:

Tween™ 80................................................................................1.0mL

Cottonseed oil or olive oil......................................................100.0mL

Preparation of Lipoidal Emulsion: Add Tween™ 80 to 400.0mL

of warm distilled/deionized water. Mix thoroughly. Add 100.0mL of

cottonseed or olive oil. Emulsify in a blender. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Add components, except lipoidal emul

sion, to distilled/deionized water and bring volume to 970.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 30.0mL of

sterile lipoidal emulsion. Mix thoroughly. Pour into sterile Petri dishes

while shaking flask to keep emulsion dispersed.

Use: For the detection, enumeration, and study of lipolytic microor

ganisms.