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Spirillum volutans Defined Medium

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

BES (N,N-bis[2-hydroxyethyl]-2-aminoethane

sulfonic acid) buffer ............................................................. 1.07g

MgSO4·7H2O ................................................................................ 1.0g

(NH4)2SO4..................................................................................... 1.0g

Succinic acid ................................................................................. 1.0g

L-Histidine..................................................................................... 0.2g

L-Isoleucine ................................................................................... 0.2g

L-Methionine ................................................................................. 0.2g

L-Threonine ................................................................................... 0.2g

NaCl .......................................................................................... 0.085g

L-Cystine ................................................................................... 0.025g

K2HPO4....................................................................................... 0.02g

FeCl3·6H2O................................................................................ 3.0mg

DL-Norepinephrine ..................................................................... 2.0mg

MnSO4·H2O............................................................................... 2.0mg

CaCO3 ........................................................................................ 1.0mg

ZnSO4·7H2O ............................................................................ 0.72mg

Na2MoO4·2H2O ..................................................................... 0.245mg

CoSO4·7H2O............................................................................ 0.14mg

CuSO4·5H2O............................................................................ 0.13mg

H2BO3 .................................................................................... 0.031mg

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Spirillum volutans.