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Sphaerotilus discophorus Medium

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 12.0g

Peptone.......................................................................................... 5.0g

MgSO4·7H2O ................................................................................ 0.2g

CaCl2........................................................................................... 0.05g

MnSO4·H2O ................................................................................ 0.05g

Ferric solution ........................................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Ferric Solution:

Composition per 100.0mL:

Ferric ammonium citrate............................................................... 0.5g

FeCl3·6H2O ................................................................................. 0.01g

Preparation of Ferric Solution: Add components to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except ferric solution,

to tap water and bring volume to 900.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add sterile ferric solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Sphaerotilus discophorus.