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Sphaerotilus Defined Medium

[所属分类:培养基配方] [发布时间:2021-8-20] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Glycerol ........................................................................................ 5.0g

Glutamic acid................................................................................ 0.9g

FeSO4·7H2O.................................................................................. 0.5g

MgSO4·7H2O................................................................................ 0.1g

CaCl2·2H2O ................................................................................ 0.03g

ZnSO4·7H2O ............................................................................... 0.03g

Phosphate solution.................................................................100.0mL

pH 7.0 ± 0.2 at 25°C

Phosphate Solution:

Composition per 500.0mL:

K2HPO4......................................................................................... 5.7g

KH2PO4......................................................................................... 2.3g

Preparation of Phosphate Solution: Add components to distilled/

deionized water and bring volume to 500.0mL. Mix thoroughly. Gently

heat until dissolved. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except phosphate solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 10 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of

sterile phosphate solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the cultivation of Sphaerotilus species.