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Soybean HiVeg Broth Base with Novobiocin

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate ........................................................................ 17.0g

NaCl.............................................................................................. 5.0g

K2HPO4......................................................................................... 4.0g

Papaic digest of soybean meal...................................................... 3.0g

Glucose ......................................................................................... 2.5g

Synthetic detergent No. I ............................................................ 1.12g

Selective solution.....................................................................10.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium, without selective novobiocin supplement, is

available as a premixed powder from HiMedia.

Selective Solution:

Composition per 10.0mL:

Novobiocin ................................................................................... 0.1g

Preparation of Selective Solution: Add novobiocin to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except selective solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Gently heat and bring to boiling. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add 10.0mL selective solution. Mix thoroughly. Distribute into

tubes or flasks. Autoclave for 5 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes.

Use: For the enrichment and isolation of Escherichia coli O157:H7

from foods.