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Soybean Extract, M-1

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soybeans..................................................................................... 50.0g

Soluble starch.............................................................................. 15.0g

(NH4)2HPO3................................................................................ 10.0g

KCl................................................................................................ 0.2g

MgSO4·7H2O................................................................................ 0.2g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add soybeans to 1.0L of distilled/deion

ized water. Soak overnight. Add 2.0g of NaOH. Adjust pH to 7.0 with

HCl. Autoclave for 60 min at 0 psi pressure–100°C. Filter through

cheesecloth. Bring volume of filtrate to 1.0L with distilled/deionized

water. Add remaining components. Mix thoroughly. Distribute into

tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Bacillus subtilis.