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SOT Medium

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

SOT Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO3...................................................................................... 16.8g

NaNO3 .......................................................................................... 2.5g

K2SO4 ........................................................................................... 1.0g

NaCl.............................................................................................. 1.0g

K2HPO4......................................................................................... 0.5g

MgSO4·7H2O................................................................................ 0.2g

Disodium EDTA·2H2O............................................................... 0.08g

CaCl2·2H2O ................................................................................ 0.04g

FeSO4·7H2O................................................................................ 0.01g

Trace metals mix A5..................................................................1.0mL

Trace metals mix B6, modified..................................................1.0mL

pH 9.0 ± 0.2 at 25°C

Trace Metals Mix A5:

Composition per liter:

H3BO3 ......................................................................................... 2.86g

MnCl2·4H2O ............................................................................... 1.81g

Na2MoO4·2H2O .......................................................................... 0.39g

ZnSO4·7H2O ............................................................................. 0.222g

CuSO4·5H2O............................................................................. 0.079g

Co(NO3)2·6H2O ........................................................................ 0.049g

Preparation of Trace Metals Mix A5: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Trace Metals Mix B6, Modified:

Composition per liter:

NH4NO3 ...................................................................................... 0.23g

K2Cr2(SO4)4·24H2O.................................................................. 0.096g

NiSO4·7H2O.............................................................................. 0.048g

Ti2(SO4)3..................................................................................... 0.04g

Na2WO4·2H2O.......................................................................... 0.018g

Preparation of Trace Metals Mix B6, Modified: Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 9.0.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and maintenance of Spirulina maxima and

Spirulina platensis.