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Sorogena Medium

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

Sorogena Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 2000.0mL:

HI Agar .........................................................................................1.0L

HI/LV Broth ..................................................................................1.0L

HI Agar:

Composition per liter:

Agar ............................................................................................ 15.0g

Hay infusion broth ........................................................................1.0L

pH 6.5 ± 0.2 at 25°C

Hay Infusion Broth:

Composition per liter:

Hay................................................................................................ 2.5g

Preparation of Hay Infusion Broth: Add hay to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly. Gently heat and

bring to boiling. Boil for 30 min. Filter through Whatman #1 filter pa

per. Bring volume to 1.0L with distilled/deionized water.

Preparation of HI Agar: Add agar to hay infusion broth and bring

volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 with 5% lactic acid

or 1N NaOH. Gently heat and bring to boiling. Autoclave for 20 min at

15 psi pressure–121°C. Pour into sterile Petri dishes.

HI/LY Broth:

Composition per liter:

Lactose .......................................................................................... 0.2g

Yeast extract.................................................................................. 0.1g

Hay infusion broth ........................................................................1.0L

pH 6.0 ± 0.2 at 25°C

Hay Infusion Broth:

Composition per liter:

Hay................................................................................................ 2.5g

Preparation of Hay Infusion Broth: Add lactose and yeast ex

tract to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Gently heat and bring to boiling. Boil for 30 min. Filter through

Whatman #1 filter paper. Bring volume to 1.0L with distilled/deionized

water.

Preparation of HI/LY Broth: Add components to 1.0L hay infu

sion broth L. Mix thoroughly. Adjust pH to 6.0 with 5% lactic acid. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically add 15.0mL of HI/LY broth

as an overlay over the surface of the HI agar plates.

Use: For the cultivation of Sorogena stoianovitchae.