Amies Transport Medium without Charcoal

[所属分类:培养基配方] [发布时间:2019-10-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Amies Transport Medium without Charcoal
Composition per liter:
NaCl..............................................................................................8.0g
Agar ..............................................................................................3.6g
Na 2 HPO 4 .....................................................................................1.15g
Sodium thioglycolate....................................................................1.0g
KCl................................................................................................0.2g
CaCl 2 ·2H 2 O...................................................................................0.1g
MgCl 2 ·6H 2 O..................................................................................0.1g
KH 2 PO 4 .........................................................................................0.2g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into flasks or tubes. Autoclave for 20 min at 15
psi pressure–121°C.
Use: For the transport of swab specimens to prolong the survival of
microorganisms, especially Neisseria gonorrhoeae, between collec-
tion and culturing.