Amies Modified Transport Medium with Charcoal

[所属分类:培养基配方] [发布时间:2019-10-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Amies Modified Transport Medium with Charcoal
Composition per liter:
Charcoal......................................................................................10.0g
Agar..............................................................................................4.0g
NaCl..............................................................................................3.0g
Na 2 HPO 4 .....................................................................................1.15g
Sodium thioglycolate....................................................................1.0g
KCl................................................................................................0.2g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O ..................................................................................0.1g
MgCl 2 ·6H 2 O .................................................................................0.1g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into flasks or tubes. Autoclave for 20 min at 15
psi pressure–121°C. While cooling, turn tubes to uniformly suspend
charcoal.
Use: For the transport of swab specimens to prolong the survival of
microorganisms, especially Neisseria gonorrhoeae, between collec-
tion and culturing. Addition of charcoal to this medium neutralizes
metabolic products that may be toxic to Neisseria gonorrhoeae.